| POLYMERS Vol.64 No.9 |
| >> Japanese |
COVER STORY Super-Resolution Fluorescence Microscopy and Polymers |
| The Nobel Prize for Chemistry in 2014 was awarded for the development of super-resolved fluorescence microscopy. For more than a century the resolution in fluorescence microscopy, and optical microscopy in general, has been limited by the diffraction property of light. As described by Ernst Abbe, the diffraction-limited resolution is about half the wavelength of light, that is, several hundreds of nm. Super-resolved fluorescence microscopy overcomes this limitation by using several independent techniques. One technique, abbreviated STORM or PALM, is based on the precise localization of the position of single fluorescent molecules, which can be alternately activated and deactivated by various physico-chemical processes. Another technique, commonly called STED, minimizes the diffraction limited excitation spot by an additional photophysical process, the stimulated emission. Super-resolution can be also achieved by a careful shaping of the excitation light in the structured-illumination technique. This special issue introduces the principles of these methods and the emerging applications in the field of polymer physics, nanotechnology, as well as examples from biological studies. It is the hope of the editors that this special issue will reveal the potential of the methods and stimulate their further use in the various fields of polymer science and technology. Guest Editor: Martin VACHA Editors: ASAMUMA, TAI, HASEGAWA, and FURUKAWA |
| Digest for English Readers | 566
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| Hot Topics in Polymer Science in SPSJ | 570
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| Commentary |
| Will Superman win the Nobel Prize? | Satoshi KAWATA | 571
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| COVER STORY: Highlight Reviews |
| Photoswitchable Fluorescent Proteins for PALM: Molecular Basis and Tips for Labeling | Hideaki MIZUNO | 572
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| Super-Resolution Localization Microscopy Reveals Nanoscale Dynamics of Polymers and Soft Matter | Shuzo HIRATA, Syoji ITO, Martin VACHA, Hiroshi MIYASAKA |
575 |
| Conformational Analysis of Polymer Chain by Single Molecule Localization Microscopy | Hiroyuki AOKI | 578 |
| PolyMANGA | 581 |
| COVER STORY: Topics and Products |
| Principle and Application of STED Microscopy | Jun-ichi HOTTA | 582 |
| Super-Resolution Fluorescence Imaging Based on Molecular Adsorption | Satoshi HABUCHI | 584 |
| The Principle and the Applications of Structured Illumination Super Resolution Microscopy | Yoshiro OIKAWA | 586 |
| IR Super-resolution Microscopy | Makoto SAKAI, Masaaki FUJII | 588 |
| Polymer Science and I: A Personal Account |
| Passive but Positive | Takuya NAKASHIMA | 590
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| Messages: “Work and Life” |
| Picasso | Miki HASEGAWA | 591
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| Front-Line Polymer Science |
| Synthesis of Inorganic Hollow Nanoparticles Templated by Polymeric Micelles | Kenichi NAKASHIMA | 592
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| From My Bookshelf | Taisei NISHIMI / Takamasa SAKAI | 598 |
| PolySCHOLA | Masayoshi OKUBO | 599 |
| Messages from SPSJ | 603 |
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