| 高分子 Vol.60 No.10 |
| >> Chinese | >> English | >> Japanese | >> Korean |
特集 バイオポリマーインターフェース
－生体分子が拓く新たな高分子界面－
|
| 展望 COVER STORY: Highlight Reviews |
| 表面修飾による生体反応のコントロール Surface Modification to Control Biological Responses |
有馬 祐介・寺村 裕治・岩田 博夫 Yusuke ARIMA, Yuji TERAMURA, and Hiroo IWATA |
| ＜要旨＞ 人工臓器や再生医療技術を用いて治療を行うとき，細胞と材料間および細胞と細胞間の相互作用の制御が要求される。本稿では異なる官能基を有するアルカンチオールの自己組織化単分子膜をモデル表面として用いて，人工材料と細胞との相互作用について得た知見をまとめた。さらに細胞表面の修飾法を用いて，再生医療の開発で求められる細胞－細胞間の相互作用を制御した新たな成果についても紹介する。 Keywords: Biological Response / Protein / Cell / Alkanethiol / Self-Assembled Monolayer / Regenerative Medicine / Amphiphilic Polymer |
| ページトップへ▲ |
| タンパク質吸着／非吸着をナノレベルで制御したバイオインターフェースへの細胞接着挙動 Cell Adhesion Behavior on the Phase Separated Biointerface Adsorbed/Nonadsorbed Protein Regulated by Nanoscale |
高井 まどか・柴山 崇・徐 知勲・石原 一彦 Madoka TAKAI, Takashi SHIBAYAMA, Ji-Hun SEO, and Kazuhiko ISHIHARA |
| ＜要旨＞ 化学組成がほぼ同様で表面形状が異なる親・疎水性ブロックコポリマーと，ランダムコポリマー表面での細胞接着挙動を解析した。ブロックコポリマーのミクロ相分離構造表面では，ランダムコポリマーの均一表面と比較してタンパク質の吸着量に差異はなかったが，細胞接着数が多かった。これは吸着タンパク質の分布の違いによるものと考察される。 Keywords: Block Copolymer / Random Copolymer / Microphase Separation / Cell Adhesion / Protein Adsorption |
| ページトップへ▲ |
| 細胞表層上のナノ糖鎖の構造・機能および精密集積化 Structure and Function of Glycoconjugates in the Membranes |
森 俊明 Toshiaki MORI |
| ＜要旨＞ 細胞表層に提示されている糖鎖は界面を介して広範囲にわたる相互作用に関わっている。最近，網羅的な解析の進歩もありその構造・機能の解明が格段に進んでいるが，まだ分子レベルでの理解や糖鎖生成メカニズムには不明な点が多い。最近の研究例を通して問題点と見通しを紹介する。 Keywords: Atomic Force Microscopy (AFM) / Verotoxin / Gb3 Sugar Molecules / Single Molecular Measurement / Glycosaminoglycan |
| ページトップへ▲ |
| トピックス COVER STORY:Topics and Products |
| 生体高分子の界面化学 抗体固定化界面での細胞ローリングの再現と幹細胞分離への応用 Stem Cell Rolling and Their Purification on an Antibody-Immobilized Column |
山岡 哲二・馬原 淳 Tetsuji YAMAOKA and Atsushi MAHARA |
| ＜要旨＞ We have recently developed a novel stem cell separation column on the basis
of cell rolling phenomena widely observed in our body. Mouse mesenchymal
stem cells are rolling on the anti CD34 (or anti CD90) antibody-immobilized
surface at a rolling rate determined by the cell surface CD34 (CD90)
density. With this system, we succeeded to purify stem cells with specific
cell differentiation features. Keywords: Mesenchymal Stem Cell / Cell Purification / CD34 / CD90 / Cell Differentiation / Cell Rolling |
| ページトップへ▲ |
| 葉緑体の再構成・組織化および光エネルギー変換材料への展開 Photoenergy Conversion Materials Based on Restructuring and Assembly of Chloroplasts |
天尾 豊 Yutaka AMAO |
| ＜要旨＞ In
chloroplasts, the photosynthetic thylakoid membranes of vascular plants
and some green algae are structurally inhomogeneous. Thylakoid membranes
in chloroplasts have two important sites, photosystem I (the oxygen evolving
site based on water photolysis) and II (photoreduction of NADP+ site).
Thus, chloroplast is a candidate for a visible light induced energy conversion
material based on water photolysis. In this paper, photoenergy conversion
materials based on the restructuring and assembly of Chloroplasts are
introduced. Keywords: Artificial Photosynthesis / Photovoltaic Conversion / Chloroplast / Water Oxidation / Biofuel Cell / Oxygen Evolved Complex |
| ページトップへ▲ |
| バクテリオナノファイバーの細胞接着機能と応用 Function of Adhesive Bacterionanofiber and Its Application |
堀 克敏 Katsutoshi HORI |
| ＜要旨＞ Many
kinds of bacteria adhere to solid surfaces through bacterionanofibers
and form biofilms. On the highly adhesive toluene-degrading bacterium Acinetobacter sp. Tol 5, we found a new proteinaceous nanofiber that
belongs to the trimeric autotransporter adhesin family. Here, its unique
properties are described after reviewing the fundamental mechanisms of
microbial adhesion. Keywords: Biofilm / Adhesion / DLVO Theory / Bacterionanofiber / Adhesin / Autotransporter / Acinetobacter / Microbial Immobilization |
| ページトップへ▲ |
| ナノ薄膜を用いた細胞の界面制御によるハイブリッド血管組織の創製 3D-Hybrid Artery Models Prepared by Cell Surface Control Using Nanofilms |
松崎 典弥・明石 満 Michiya MATSUSAKI and Mitsuru AKASHI |
| ＜要旨＞ Three-dimensional
(3D) analysis of nitric oxide (NO) diffusion in blood vessels in response
to drug stimulation was achieved using 3D-artery models including NO
sensor particles. This method would be useful as in vitro bioassays of
tissue responses instead of animal experiments. Keywords: Artery Models / Biosensors / Nitric Oxide / Layer-by-Layer / Pharmaceutical Assays |
| ページトップへ▲ |
| ポリスチレン親和性ペプチドを利用したタンパク質固定化技術の開発 Development of Protein Immobilization Method by Use of Polystyrene-Binding Peptide |
熊田 陽一 Yoichi KUMADA |
| ＜要旨＞ In
this study we report the development of a novel protein immobilization
method by the use of affinity peptides that specifically bind to the
surfaces of hydrophilic polystyrene (phi-PS). A PS-tag-fused enzyme and
a single-chain Fv antibody were site-specifically immobilized onto the
surface of a phi-PS plate, while highly maintaining their biological
activities. Keywords: Polystyrene-Binding Peptide / Protein Immobilization / Polystyrene / Antibody / Immunoassay / Single-Chain Fv / Solid-Phase Refolding |
| ページトップへ▲ |
| バイオサーファクタントの機能利用 Functional Development of Glycolipid Biosurfactants |
福岡 徳馬・森田 友岳・井村 知弘・北川 優・北本 大 Tokuma FUKUOKA, Tomotake MORITA, Tomohiro IMURA, Masaru KITAGAWA, and Dai KITAMOTO |
| ＜要旨＞ Mannosylerythritol
lipids (MELs) are one of the most promising glycolipid-type biosurfactants,
which are amphiphilic compounds produced by microorganisms from renewable
resources. MELs not only exhibit excellent interfacial and self-assembling
properties but also show versatile biological actions. MELs should thus
be considered novel functional bio-based materials, and their broader
applications in various advanced technologies should be investigated. Keywords: Biosurfactant / Mannosylerythritol Lipid / Glycolipid / Yeast / Microbial Production / Protein Separation / Functional Moisturizer / Cosmetics |
| ページトップへ▲ |
| 高分子科学最近の進歩 Front-Line Polymer Science |
| 生体高分子をキラル反応場として活用した超分子不斉光化学系の創製 －環境調和型不斉合成法の開発を目指して－ Supramolecular Asymmetric Photochirogenesis with Biopolymers as a Chiral Confined Reaction Media |
和田 健彦 Takehiko WADA |
| ＜要旨＞ Asymmetric
photochemistry provides us with new versatile routes to novel/strained
chiral compounds. Recently, much effort has been devoted to the supramolecular
photochirogenesis, exploiting the chiral environment of natural and synthetic
hosts, such as cyclodextrin, modified zeolite, ds-DNA and protein. Photochirogenesis
with biomolecules is particularly attractive and advantageous in view
of the inherently chiral, finely-defined 3D structures. Keywords: Photochemistry / Asymmetric Synthesis / Supramolecule / Biopolymer / Protein / DNA / Antibody / Supramolecular Asymmetric Photochirogenesis |
| ページトップへ▲ |
| Copyright(C) 2010 The Society of Polymer Science, Japan All Rights Reserved. |